Codeofchina.com is in charge of this English translation. In case of any doubt about the English translation, the Chinese original shall be considered authoritative.
This standard replaces GB 19191-2003 Food Additive - Natural Vitamin E.
The following main changes have been made with respect to GB 19191-2003:
——The standard name is revised as National Food Safety Standard - Food Additive - Vitamin E;
——The types of tocopherols contained in vitamin E are modified;
——Refractive index requirements are added;
——Requirements of absorption coefficient indexes are added.
National Food Safety Standard
Food Additive - Vitamin E
1 Scope
This standard is applicable to products made from the natural vitamin E extracted from the by-products of natural edible vegetable oils or synthetic vitamin E by incorporating with edible vegetable oils. This standard is applicable vitamin E containing d-α-tocopherol, dl-α-tocopherol, d-α-tocopheryl acetate, dl-α-tocopheryl acetate, d-α-tocopheryl acid succinate, dl-α-tocopheryl acid succinate, d-α-tocopheryl acetate concentrate, and concentrate of mixed tocopherols.
2 Chemical Name, Molecular Formula, Structural Formula and Relative Molecular Mass
2.1 Chemical name
d-α-tocopherol: (+) 2,5,7,8-tetramethyl-2-(4,8,12-trimethyl-tridecyl)-6-benzopyran alcohol;
dl-α-tocopherol: (+) 2,5,7,8-tetramethyl-2-(4,8,12-trimethyl-tridecyl)-6-chromanol;
d-α-tocopheryl acetate:
(+)2,5,7,8-tetramethyl-2-(4,8,12-trimethyl-tridecyl) chromanol-6-acetate;
dl-α-tocopheryl acetate:
(+)2,5,7,8-tetramethyl-2-(4,8,12-trimethyl-tridecyl)-2H-1-benzopyran-6-ol acetate;
d-α-tocopheryl acid succinate:
(+)2,5,7,8-tetramethyl-2-(4,8,12-trimethyl-tridecyl) chromanol-6-succinate;
dl-α-tocopheryl acid succinate:
(+)2,5,7,8-tetramethyl-2-(4,8,12-trimethyl-tridecyl) chromanol-6-succinate.
2.2 Molecular formula
d-α-tocopherol: C29H50O2
dl-α-tocopherol: C29H50O2
d-α-tocopheryl acetate: C31H52O3
dl-α-tocopheryl acetate: C31H52O3
d-α-tocopheryl acid succinate: C33H54O5
dl-α-tocopheryl acid succinate: C33H54O5
2.3 Structural formula
The radical groups R1, R2, R3 and R4 corresponding to structural formulae of d-α-tocopherol, dl-α-tocopherol, d-α-tocopheryl acetate, dl-α-tocopheryl acetate, d-α-tocopheryl acid succinate, dl-α-tocopheryl acid succinate, d-α-tocopheryl acetate concentrate and concentrate of mixed tocopherols are as shown in Table 1.
Table 1
Type Substituting group R1 Substituting group R2 Substituting group R3 Substituting group R4
d-α-tocopherol CH3 CH3 CH3 H
d-β-tocopherol CH3 H CH3 H
d-γ-tocopherol H CH3 CH3 H
d-δ-tocopherol H H CH3 H
dl-α-tocopherol CH3 CH3 CH3 H
d-α-tocopheryl acetate CH3 CH3 CH3 CH3CO
dl-α-tocopheryl acetate CH3 CH3 CH3 CH3CO
d-α-tocopheryl acid succinate CH3 CH3 CH3 HOCO(CH3)2CO
dl-α-tocopheryl acid succinate CH3 CH3 CH3 HOCO(CH3)2CO
2.4 Relative molecular mass
d-α-tocopherol: 430.71 (as per 2013 international relative atomic mass);
dl-α-tocopherol: 430.71 (as per 2013 international relative atomic mass);
d-α-tocopheryl acetate: 472.75 (as per 2013 international relative atomic mass);
dl-α-tocopheryl acetate: 472.75 (as per 2013 international relative atomic mass);
d-α-tocopheryl acid succinate: 530.79 (as per 2013 international relative atomic mass);
dl-α-tocopheryl acid succinate: 530.79(as per 2013 international relative atomic mass).
3 Technical Requirements
3.1 Sensory requirements
Sensory requirements shall be in accordance with those specified in Table 2.
Table 2 Sensory Requirements
Item Requirements Inspection method
Color and lustre d-α-tocopherol: red brown to pale yellow Place a proper amount of specimen into a white porcelain dish or transparent beaker, and then observe its color and lustre as well as state under natural light.
d-α-tocopheryl acetate: colorless to yellow
d-α-tocopheryl acetate concentrate: light brown to pale yellow
d-α-tocopheryl acid succinate: white to off-white
dl-α-tocopheryl acid succinate: white to off-white
Concentrate of mixed tocopherols: red brown to pale yellow
Character d-α-tocopherol: clear oily liquid
d-α-tocopheryl acetate: clear viscous oily liquid, solidify when keeping still and melt at about 25℃
d-α-tocopheryl acetate concentrate: clear viscous oily liquid
d-α-tocopheryl acid succinate: crystalline powder, melt at about 75℃
dl-α-tocopheryl acid succinate: crystalline powder, melt at about 75℃
Concentrate of mixed tocopherols: clear oily liquid, allow the separation of tiny crystal
3.2 Physical and chemical indexes
Physical and chemical indexes shall be in accordance with those specified in Table 3.
?
Table 3 Physical and Chemical Indexes
Item Index Inspection method
d-α-tocopherol d-α-tocopheryl acetate concentrate d-α-tocopheryl acetate d-α-tocopheryl acid succinate dl-α-tocopheryl acid succinate Concentrate of mixed tocopherols
E50 type E70 type
Contenta, ω/% Total tocopherols ≥50.0 ≥70.0 ≥70.0 96.0 ~ 102.0. ≥50.0 A.3 of
Annex A
Where,
d-α-tocopherol ≥ 95.0 —
d-β-, d- γ- and d–e-tocopherols — ≥ 80.0
pH/mL ≤ 1.0 ≤ 0.5 18.0 ~ 19.3 ≤ 1.0 A.4 of
Annex A
Specific rotation ≥ + 24° — + 20° A.5 of
Annex A
Absorption coefficient
(284nm) — 41.0~45.0 — A.6 of
Annex A
Refractive index
— 1.494~1.499 — GB/T 614
Heavy metal (calculated by Pb)/
(mg/kg) ≤ 10 GB/T 5009.74
Note 1: By taking vitamin E complying with this standard as raw material, commercial products of food additive vitamin E shall be made of food raw materials such as food additives complying with quality specification standard like antioxidant, emulsifier, thickener, anti-caking agent and (or) edible vegetable oils; among which, the content in d-α-tocopherol, d-α-tocopheryl acetate, d-α-tocopheryl acetate concentrate, d-α-tocopheryl acid succinate, dl-α-tocopheryl acid succinate, concentrate of mixed tocopherols shall conform to the marking values.
Note 2: Technical indexes of dl-α-tocopherol shall meet the relevant requirements of GB 29942, the technical indexes of dl-α-tocopheryl acetate shall meet the relevant requirements of GB 14756.
a The content may also be expressed in international unit (IU/g), 1mg d-α-tocopherol=1.49 IU, 1mg d-α-tocopheryl acetate=1.36 IU, 1mg d-α-tocopheryl acid succinate=1.21 IU.
Annex A
Inspection Methods
A.1 General requirements
Unless otherwise specified in this standard, the purity of the adopted reagents shall be above analytically pure; the adopted standard titration solutions, standard solutions for impurity determination, preparations and products shall be prepared according to the requirements of GB/T 601, GB/T 602 and GB/T 603; the test water shall be Grade 3 water specified in GB/T 6682. Solutions used in the test are all aqueous solution, unless other solvents are indicated to be used for preparation.
Foreword i
1 Scope
2 Chemical Name, Molecular Formula, Structural Formula and Relative Molecular Mass
3 Technical Requirements
Annex A Inspection Methods
