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National Food Safety Standard
Food Additive - 5'-Cytidylic Acid Disodium Salt
1 Scope
This standard is applicable to food additive - 5'-cytidylic acid disodium salt.
2 Chemical Formula, Structural Formula and Relative Molecular Mass
2.1 Chemical formula
C9H12N3Na2O8P
2.2 Structural formula
2.3 Relative molecular mass
367.16 (as per 2007 international relative atomic mass)
3 Technical Requirements
3.1 Sensory requirements
Sensory requirements shall be in accordance with those specified in Table 1.
Table 1 Sensory Requirements
Item Requirement Inspection method
Color and lustre Colorless to white White Put a proper amount of specimen into a clean and dry white porcelain dish, observe its color and lustre as well as state under natural light, and smell its odor.
State Crystal Crystalline powder
Odor With special odor
3.2 Physical and chemical indexes
Physical and chemical indexes shall be in accordance with those specified in Table 2.?
Table 2 Physical and Chemical Indexes
Item Index Inspection method
Content of 5'-cytidylic acid disodium salt (C9H12N3Na2O8P) (calculated by dry basis), ω/% 97.0~102.0 A.3 of Annex A
pH 8.0~9.5 GB/T 9724
Moisture content, ω/% ≤ 26.0 GB/T 6283 a
Heavy metal (calculated by Pb)/(mg/kg) ≤ 20 GB 5009.74
Arsenic (As)/(mg/kg) ≤ 3.0 GB 5009.76
Clarity Pass the test A.4 of Annex A
Absorbance ratio 250nm/260nm 0.40~0.52 A.5 of Annex A
280nm/260nm 1.85~2.20
Other nucleic acid analytes Pass the test A.6 of Annex A
a Weigh 0.15g of specimen and take back titration method as the determination method.
Annex A
Inspection Methods
A.1 General requirements
Unless otherwise specified, analytically pure reagents and Grade 3 water (specified in GB/T 6682) shall be used in this standard; if there is no requirement, standard solution, the standard solution for impurity determination, preparations and products used in test shall be prepared according to those specified in GB/T 601, GB/T 602 and GB/T 603. All solutions used in the test are aqueous solution, unless other solvents are indicated to be used for preparation.
A.2 Identification test
A.2.1 Reagents and materials
A.2.1.1 Hydrochloric acid.
A.2.1.2 Nitric acid.
A.2.1.3 Hydrochloric acid solution: 1→1 000.
A.2.1.4 Saturated bromine solution.
A.2.1.5 3,5-vesorcinol-ethanol solution: 1→10.
A.2.1.6 Ammonium ferric sulfate-hydrochloric acid solution: 1→1 000.
A.2.1.7 Magnesium oxide test solution.
A.2.1.8 Sodium hydroxide solution 40g/L.
A.2.1.9 Sodium hydroxide solution: 1→25.
A.2.1.10 Ammonia solution: 2+3.
A.2.1.11 Ammonium molybdate solution: weigh 6.5g of molybdenum trioxide powder, add into ammonia solution (14.5+14) to dissolve it. After cooling, add nitric acid solution (32+40) while stirring, then filter the solution with asbestos after it is placed for 48h. This solution cannot be preserved for a long-time. Measure 5mL of the solution, add into 2mL of sodium hydrogen phosphate solution (1→8), at the time a large amount of yellow sedimentation shall be generated immediately or after the solution is heated slightly, otherwise the solution is unavailable. Preserve the solution in a dark place, if any sedimentation forms, use the supernatant liquid.
1 Scope
2 Chemical Formula, Structural Formula and Relative Molecular Mass
3 Technical Requirements
Annex A Inspection Methods
